man if you have .iso file protected as dvd.. just open it windows media player.. thats it.. it will show you the chapter & will work as normal dvd as how you insert any other dvd in dvd player..
just use vlc mediaplayer http://www.videolan.org/vlc/
most DVD?s are DVD5, so they would fit Haven’t come ascors many in the last decade. This might be a regional difference if American DVDs typically only come with one language, minus extras, etc.
VLC Media Player like others said - open the ISO file from VLC and it plays like a DVD--no extra work
Playing a .iso without burning to a DVD